4LKV
Determinants of lipid substrate and membrane binding for the tetraacyldisaccharide-1-phosphate 4 -kinase LpxK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 136.632, 140.691, 173.846 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.510 - 3.511 |
R-factor | 0.273 |
Rwork | 0.267 |
R-free | 0.33050 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.833 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 9.480 | 3.500 |
Rmerge | 0.089 | 0.687 | |
Number of reflections | 20053 | ||
<I/σ(I)> | 22.4 | 2 | |
Completeness [%] | 94.2 | 96.9 | 74.2 |
Redundancy | 12.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES, MPD, lipid IVa, Tris, Triton X-100, DDM, NaCl, MgCl2, ADP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |