4LKM
Crystal structure of Plk1 Polo-box domain in complex with PL-74
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.600, 82.000, 151.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.630 - 2.000 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.073 | 0.039 | 0.346 |
| Number of reflections | 33797 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.8 | 99.9 | 99 |
| Redundancy | 6.4 | 6.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | 0.2M sodium citrate, 500mM ammonium sulfate, pH 4.0, vapor diffusion, temperature 293K |
