4LKL
Crystal structure of Plk1 Polo-box domain in complex with PL-55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.340, 50.940, 57.000 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 37.010 - 1.580 |
| Rwork | 0.224 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c5l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
| Rmerge | 0.051 | 0.029 | 0.476 |
| Number of reflections | 28953 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 99.5 | 98.5 | 98.3 |
| Redundancy | 3.6 | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.2M calcium acetate, 20% PEG3350, pH 7.5, vapor diffusion, temperature 293K |
