4LK3
Crystal structure of Human UDP-xylose synthase R236A substitution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.570, 92.070, 290.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.370 - 2.640 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b69 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.092 | 0.866 |
| Number of reflections | 65871 | |
| <I/σ(I)> | 19.9 | 2.7 |
| Completeness [%] | 98.4 | 86.2 |
| Redundancy | 11.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 293 | 0.1 M ammonium sulfate, 0.1 M succinate, 10% DMSO, pH 5.4, Vapor diffusion, hanging drop, temperature 293K |
