4LJP
Structure of an active ligase (HOIP-H889A)/ubiquitin transfer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 31 |
| Unit cell lengths | 46.000, 46.000, 133.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.170 - 2.150 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ljo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data reduction software | CCP4 |
| Data scaling software | CCP4 |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.170 |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.094 |
| Number of reflections | 17108 |
| <I/σ(I)> | 9.3 |
| Completeness [%] | 99.5 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M amino acids, 0.1 M imidazole, MES, 30 % P550 MME_P20K , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
