4LIU

Structure of YcfD, a Ribosomal oxygenase from Escherichia coli.

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	75.110, 75.110, 209.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.024 - 2.700
R-factor	0.1986
Rwork	0.197
R-free	0.23730
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.807
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	SHARP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.721	43.024	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.029	0.872
Number of reflections	17365
<I/σ(I)>	11	19	0.9
Completeness [%]	99.9	98.4	100
Redundancy	6.1	4.9	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	50mM Tris.HCl pH 7.5, 0.4M Ammonium dihydrogen phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 285K