4LG4
Structural Basis for Autoactivation of Human Mst2 Kinase and Its Regulation by RASSF5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.179, 69.771, 148.082 |
| Unit cell angles | 90.00, 108.61, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.420 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3com |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.645 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.342 | 2.460 |
| High resolution limit [Å] | 2.415 | 2.420 |
| Rmerge | 0.052 | 0.537 |
| Number of reflections | 83850 | |
| <I/σ(I)> | 27.4 | 1.7 |
| Completeness [%] | 99.5 | 91.2 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 277 | 0.2 M sodium citrate, 15% (w/v) PEG 3350, 0.1 M HEPES, pH 7.7, vapor diffusion, hanging drop, temperature 277K |
