4LG2

Crystal structure of Reston Ebola virus VP35 RNA binding domain bound to 12-bp dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	80
Detector technology	CCD
Collection date	2012-08-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	P 21 21 21
Unit cell lengths	55.090, 72.871, 175.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.296 - 2.700
R-factor	0.2112
Rwork	0.209
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ks8
RMSD bond length	0.007
RMSD bond angle	1.061
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.079	0.506
Number of reflections	20077
<I/σ(I)>	8.5	1.9
Completeness [%]	100.0	100
Redundancy	4.36	4.49

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	298	0.2 M Li2SO4, 0.1 M phosphate-citrate, pH 4.2 and 20% (w/v) PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 298K