4LF3
Inhibitory Mechanism of an Allosteric Antibody Targeting the Glucagon Receptor
Experimental procedure
| Experimental method | SINGLE |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-25 |
| Detector | ADSC |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.928, 62.777, 116.432 |
| Unit cell angles | 90.00, 106.04, 90.00 |
Refinement procedure
| Resolution | 55.950 - 2.735 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ers |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.538 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.950 | 55.950 | 2.880 |
| High resolution limit [Å] | 2.735 | 8.650 | 2.735 |
| Rmerge | 0.022 | 0.367 | |
| Total number of observations | 3726 | 13662 | |
| Number of reflections | 35933 | ||
| <I/σ(I)> | 12.6 | 27.1 | 2.1 |
| Completeness [%] | 92.6 | 88.4 | 92.4 |
| Redundancy | 2.9 | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1 M Tris-HCl, 0.2 M NaCl, PEG3350, pH 8.5, vapor diffusion, hanging drop, temperature 289K |
