4LEZ
Structure of mouse cGAS bound to an 18bp DNA and cGAS product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 153 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.209, 99.031, 142.631 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.549 - 2.360 |
R-factor | 0.1946 |
Rwork | 0.192 |
R-free | 0.24660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.229 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 | 2.430 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.069 | |
Number of reflections | 45447 | |
Completeness [%] | 96.6 | 97 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |