4LEO

Crystal structure of anti-HER3 Fab RG7116 in complex with the extracellular domains of human Her3 (ERBB3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 1
Unit cell lengths	54.458, 59.359, 103.661
Unit cell angles	97.64, 106.85, 96.83

Refinement procedure

Resolution	48.830 - 2.640
R-factor	0.2295
Rwork	0.228
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.764
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	2.800
High resolution limit [Å]	2.640	2.640
Number of reflections	34229
Completeness [%]	95.6	93.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350 , pH pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K