4LCF

Crystal structure of the Pseudomonas aeruginosa LPXC/LPC-014 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-06-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.193, 73.819, 88.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.293 - 1.599
R-factor	0.1922
Rwork	0.191
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.938
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.599	4.340	1.599
Rmerge	0.042	0.032	0.453
Number of reflections	42896
<I/σ(I)>	20.3
Completeness [%]	91.9	99.1	100
Redundancy	5.5	5.7	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1