4LC9
Structural Basis for Regulation of Human Glucokinase by Glucokinase Regulatory Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.663, 105.312, 132.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.929 - 3.400 |
| R-factor | 0.2461 |
| Rwork | 0.244 |
| R-free | 0.29220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.071 | 0.699 |
| Number of reflections | 18946 | |
| <I/σ(I)> | 12.93 | 1.73 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.5 | 6.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | Crystals were obtained at 20 C by mixing 200 nL of GCK-GKRP-F6P complex solution with 100 nL of reservoir solution containing 0.1 M HEPES, pH 7.1, and 8% w/v PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
