4L9M
Autoinhibited state of the Ras-specific exchange factor RasGRP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.380, 76.380, 408.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.435 - 3.000 |
| R-factor | 0.2266 |
| Rwork | 0.224 |
| R-free | 0.26910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2IJE and 1BKD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.440 | 47.440 | 3.240 |
| High resolution limit [Å] | 3.000 | 7.940 | 3.000 |
| Number of reflections | 15253 | ||
| Completeness [%] | 99.7 | 97.1 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.15 M sodium citrate tribasic, 22% PEG 3350, 1 mM MnCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
