4L9I
Bovine G Protein Coupled Receptor Kinase 1 in Complex with Paroxetine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-21 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.788, 122.089, 152.892 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.320 |
| R-factor | 0.18833 |
| Rwork | 0.186 |
| R-free | 0.23053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.377 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 6.290 | 2.320 |
| Rmerge | 0.109 | 0.038 | 0.949 |
| Number of reflections | 54845 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 9.1 | 8.4 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 298 | 12% PEG3350, 1M NaCl, pH 5.75, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
