4L8R
Structure of mrna stem-loop, human stem-loop binding protein and 3'hexo ternary complex
Replaces: 4HXHExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.758, 90.802, 128.636 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.930 - 2.600 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.24900 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (THEN SOLVE/RESOLVE) |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.085 | 0.439 |
Number of reflections | 55893 | |
<I/σ(I)> | 15.1 | 3.1 |
Completeness [%] | 98.9 | 96.5 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 8% (W/V) TACSIMATE (PH 6.0), 18% (W/V) PEG3350, MACROSEEDING, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, VAPOR DIFFUSION, HANGING DROP |