4L6T
GM1 bound form of the ECX AB5 holotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953699 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 120.441, 120.441, 273.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.818 - 1.859 |
| R-factor | 0.1791 |
| Rwork | 0.178 |
| R-free | 0.19840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l63 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.043 | 0.388 |
| Number of reflections | 107527 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.8 M NaH2PO4, 0.8 M KH2PO4, 0.1 M Na Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
