4L4J

Crystal structure of fc-fragment of human IgG2-Sigma antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-29
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 21
Unit cell lengths	68.540, 69.970, 112.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.920
R-factor	0.20085
Rwork	0.200
R-free	0.24652
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4haf
RMSD bond length	0.010
RMSD bond angle	1.288
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.059	0.351
Number of reflections	40887
<I/σ(I)>	22	3.2
Completeness [%]	97.2	72.9
Redundancy	6.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M SODIUM ACETATE, 18% PEG 3350, 0.2 M SODIUM FORMATE. CRYO CONDITIONS: 0.1 M SODIUM ACETATE, 20% PEG 3350, 0.2 M SODIUM FORMATE, 20% GLYCEROL, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K