4L4J
Crystal structure of fc-fragment of human IgG2-Sigma antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-29 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.540, 69.970, 112.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.920 |
R-factor | 0.20085 |
Rwork | 0.200 |
R-free | 0.24652 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4haf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.288 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.351 |
Number of reflections | 40887 | |
<I/σ(I)> | 22 | 3.2 |
Completeness [%] | 97.2 | 72.9 |
Redundancy | 6.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M SODIUM ACETATE, 18% PEG 3350, 0.2 M SODIUM FORMATE. CRYO CONDITIONS: 0.1 M SODIUM ACETATE, 20% PEG 3350, 0.2 M SODIUM FORMATE, 20% GLYCEROL, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |