4L4I
Crystal structure of mouse Ryanodine Receptor isoform 2 (RyR2) 1-547 disease mutant R420Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 78.050, 78.050, 248.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.890 - 2.150 |
| R-factor | 0.23006 |
| Rwork | 0.228 |
| R-free | 0.26287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l4h |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 6.800 | 2.150 |
| Rmerge | 0.103 | 1.888 | |
| Number of reflections | 43007 | ||
| <I/σ(I)> | 20.2 | 2.4 | |
| Completeness [%] | 99.7 | 94.1 | 99.7 |
| Redundancy | 14.3 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris, pH 8.15, 5-10% PEG3350, 1% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
