4L4I

Crystal structure of mouse Ryanodine Receptor isoform 2 (RyR2) 1-547 disease mutant R420Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 42 21 2
Unit cell lengths	78.050, 78.050, 248.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 2.150
R-factor	0.23006
Rwork	0.228
R-free	0.26287
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l4h
RMSD bond length	0.013
RMSD bond angle	1.430
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.270
High resolution limit [Å]	2.150	6.800	2.150
Rmerge	0.103		1.888
Number of reflections	43007
<I/σ(I)>	20.2		2.4
Completeness [%]	99.7	94.1	99.7
Redundancy	14.3		14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.1 M Tris, pH 8.15, 5-10% PEG3350, 1% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K