4L3Q

Crystal structure of glucokinase-activator complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2004-12-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	79.512, 79.512, 321.131
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.840 - 2.700
R-factor	0.2591
Rwork	0.254
R-free	0.30377
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1v4s
RMSD bond length	0.013
RMSD bond angle	1.511
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNX
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.042	0.155
Number of reflections	17045
<I/σ(I)>	32.3	9.3
Completeness [%]	97.4	99.8
Redundancy		4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	VAPOR DIFFUSION, HANGING DROP, temperature 293K