4L01

Crystal structure of the V658F apo Jak1 pseudokinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	47.288, 80.557, 76.555
Unit cell angles	90.00, 95.29, 90.00

Refinement procedure

Resolution	41.860 - 1.900
R-factor	0.20355
Rwork	0.202
R-free	0.23266
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.998
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	41195
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP