4KRP
Nanobody/VHH domain 9G8 in complex with the extracellular region of EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.372, 95.841, 129.481 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 44.460 - 2.823 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YY9 and 4KRN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.926 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.096 | 0.039 | 0.413 |
| Number of reflections | 37840 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.0 | 99.2 | 92.4 |
| Redundancy | 3.7 | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% PEG3350, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
