4KQ8
Structure of Recombinant Human Cytochrome P450 Aromatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.821, 140.821, 116.477 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.290 |
| R-factor | 0.21735 |
| Rwork | 0.216 |
| R-free | 0.24820 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 121.950 |
| High resolution limit [Å] | 3.290 |
| Rmerge | 0.093 |
| Number of reflections | 19096 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 97.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 277 | Crystallized from 24%-30% PEG4000, 0.5 M NaCl, 0.05M Tris-HCl and vapor diffused in sealed 24-well sitting drop plates. Crystals appeared after 1-6 week of initial set-up and continued to grow for an additional 2 to 6 weeks after first appearance, pH 7.4, VAPOR DIFFUSION, temperature 277K |
