4KQ3

Crystal structure of Anti-IL-17A antibody CNTO3186

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2008-11-04
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	93.450, 60.560, 72.140
Unit cell angles	90.00, 99.66, 90.00

Refinement procedure

Resolution	15.000 - 1.920
R-factor	0.17251
Rwork	0.171
R-free	0.22523
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.034
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (SEARCH MODEL: 1RZ7 (VL))
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.035	0.150
Number of reflections	28013
<I/σ(I)>	32.8	6.6
Completeness [%]	91.7	55
Redundancy	4.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	18% PEG3000, 0.2 M ammonium acetate, 0.1 M MES, pH 6.5, cryoprotectant: 20% PEG3000, 0.16 M ammonium acetate, 0.1 M MES, PH 6.5, 24% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K