4KJY

Complex of high-affinity SIRP alpha variant FD6 with CD47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	120
Detector technology	CCD
Collection date	2011-09-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 65 2 2
Unit cell lengths	71.340, 71.340, 399.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.291 - 1.930
R-factor	0.2206
Rwork	0.219
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.089
Number of reflections	36706
<I/σ(I)>	21.4
Completeness [%]	83.1	97.3	30.3
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	295	Crystals were obtained by addition of 0.1 microL protein to an equal volume of 2.0 M ammonium sulfate and 0.1 M Tris, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K