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4KFB

HIV-1 reverse transcriptase with bound fragment at NNRTI adjacent site

Replaces:  4I7G
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]100
Detector technologyCCD
Collection date2010-07-02
DetectorADSC QUANTUM 270
Wavelength(s)0.917
Spacegroup nameC 1 2 1
Unit cell lengths161.408, 73.145, 108.768
Unit cell angles90.00, 100.47, 90.00
Refinement procedure
Resolution43.297 - 1.854
R-factor0.1862
Rwork0.186
R-free0.21440
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.201
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX (1.8.1_1168)
Refinement softwarePHENIX ((phenix.refine: 1.8.2_1309))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]43.2971.880
High resolution limit [Å]1.8541.850
Rmerge0.065
Number of reflections105443
<I/σ(I)>13.79
Completeness [%]95.186.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.711% PEG 8000, 4% PEG 400, 50 MM IMIDAZOLE, 10 MM SPERMINE, 15 MM MGSO4, 100 MM AMMONIUM SULFATE, AND 5 MM TRIS(2-CARBOXYETHYL)PHOSPHINE, pH 6.7, VAPOR DIFFUSION, HANGING DROP

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