4KF6
Crystal structure of human ceramide-1-phosphate transfer protein (CPTP) in complex with 8:0 Ceramide-1-Phosphate (8:0-C1P)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.884, 122.399, 149.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.827 - 3.195 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.26460 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.997 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.086 | 0.670 |
Number of reflections | 25734 | |
<I/σ(I)> | 19.6 | 2.4 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Ammonium sulfate, 0.1 M Tris pH 8.0, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |