4KEH

Crosslinked Crystal Structure of Type II Fatty Synthase Dehydratase, FabA, and Acyl Carrier Protein, AcpP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Detector technology	PIXEL
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	84.405, 122.178, 122.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.679 - 1.901
R-factor	0.181
Rwork	0.179
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.584
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.679
High resolution limit [Å]	1.901
Number of reflections	49422

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	1M LiCl, 35% PEGS 3350, 0.35M Sodium Acetate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K