4KEH
Crosslinked Crystal Structure of Type II Fatty Synthase Dehydratase, FabA, and Acyl Carrier Protein, AcpP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 84.405, 122.178, 122.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.679 - 1.901 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.584 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.679 |
High resolution limit [Å] | 1.901 |
Number of reflections | 49422 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 1M LiCl, 35% PEGS 3350, 0.35M Sodium Acetate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |