4K94
Crystal structure of KIT D4D5 fragment in complex with anti-Kit antibody Fab19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-27 |
Detector | PSI PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.580, 48.980, 106.970 |
Unit cell angles | 90.00, 122.38, 90.00 |
Refinement procedure
Resolution | 45.495 - 2.400 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.770 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 24912 | |
<I/σ(I)> | 11 | 1.2 |
Completeness [%] | 84.5 | 64.1 |
Redundancy | 3.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 13% PEG 3350, 0.5 M magnesium chloride, 0.1 M Tris-HCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |