4K7D
Crystal Structure of Parkin C-terminal RING domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.2824 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.700, 106.625, 154.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.310 - 2.800 |
R-factor | 0.1869 |
Rwork | 0.184 |
R-free | 0.23300 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.914 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SOLVE |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.330 | 53.313 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.079 | 0.036 | 0.464 |
Total number of observations | 10817 | 48748 | |
Number of reflections | 35121 | ||
<I/σ(I)> | 19.9 | 16.1 | 1.6 |
Completeness [%] | 99.9 | 98.3 | 100 |
Redundancy | 9.7 | 8.9 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.9 M sodium malonate pH 7.0, 0.1 M HEPES pH 7.0, 5% (v/v) glycerol, 56 mM 2-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |