4K6I

Crystal structure of human retinoid X receptor alpha-ligand binding domain complex with Targretin and the coactivator peptide GRIP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-09-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.315
Spacegroup name	P 43 21 2
Unit cell lengths	68.150, 68.150, 106.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.300 - 2.100
R-factor	0.246
Rwork	0.246
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oap
RMSD bond length	0.009
RMSD bond angle	1.162
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.078
<I/σ(I)>	14.1	4.5
Completeness [%]	96.9	99.7
Redundancy	8.12	7.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	5-15% PEG4000, 6-12% Glycerol, 0.1M Bis-Tris pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K