4K4G
Ternary crystal structures of human DNA POLYMERASE LAMBDA IN COMPLEX WITH DNA AND L-DCTP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-14 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 194.570, 97.810, 105.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.600 - 2.150 |
R-factor | 0.20267 |
Rwork | 0.200 |
R-free | 0.24985 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.771 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.600 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 107569 | |
Completeness [%] | 98.2 | 93.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M SODIUM CACODYLATE, 0.2 M CALCIUM ACETATE, 4% PEG 8000 , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |