4K2R
Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-02-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.530, 53.150, 68.960 |
| Unit cell angles | 106.10, 93.26, 104.40 |
Refinement procedure
| Resolution | 49.090 - 3.000 |
| R-factor | 0.21294 |
| Rwork | 0.209 |
| R-free | 0.28695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.552 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.240 | |
| Number of reflections | 11992 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 95.6 | 94.6 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1 M Bis-Tris pH 6.5, 20% Polyethylene glycol monomethyl ether 5,000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
