4JVS

Crystal structure of LepB GAP domain from Legionella drancourtii in complex with Rab1-GDP and AlF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	200
Detector technology	CCD
Collection date	2012-11-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	95.561, 95.561, 197.724
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 2.783
R-factor	0.2256
Rwork	0.224
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.503
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.780
Number of reflections	14058
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	0.2M ammonium acetate, 0.1M Tris, 25%(w/v) polyethylene glycol 3350, pH 8.5, EVAPORATION, temperature 298.0K