4JS0

Complex of Cdc42 with the CRIB-PR domain of IRSp53

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.9769
Spacegroup name	P 42 21 2
Unit cell lengths	67.230, 67.230, 79.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.296 - 1.900
R-factor	0.1481
Rwork	0.146
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.689
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (Phaser-MR)
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	15019
Completeness [%]	100.0	100
Redundancy	17.8	18.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	18-20% PEG 4000, 0.2M magnesium chloride, 0.004M betain hydrochloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K