4JPV
Crystal structure of broadly and potently neutralizing antibody 3bnc117 in complex with hiv-1 gp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-05 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.627, 69.887, 231.633 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.966 - 2.827 |
R-factor | 0.2025 |
Rwork | 0.199 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3se9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.105 | 0.510 |
Number of reflections | 27017 | |
<I/σ(I)> | 14.1 | 2.1 |
Completeness [%] | 98.4 | 87.1 |
Redundancy | 4.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 11% PEG 8000, 7% ethylene glycol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |