4JPK
Crystal structure of the germline-targeting HIV-1 gp120 engineered outer domain eOD-GT6 in complex with a putative VRC01 germline precursor Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.260, 63.300, 61.300 |
Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
Resolution | 36.307 - 2.400 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4JPI AND 4JPJ |
RMSD bond length | 0.005 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 25368 | |
<I/σ(I)> | 9.4 | 2.1 |
Completeness [%] | 93.6 | 96.8 |
Redundancy | 2.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20% w/v PEG4000, 0.1 M sodium citrate, 20% v/v 2-propanol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |