4JHR
An auto-inhibited conformation of LGN reveals a distinct interaction mode between GoLoco motifs and TPR motifs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-12-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 72.213, 81.803, 106.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.043 - 2.800 |
| R-factor | 0.2527 |
| Rwork | 0.250 |
| R-free | 0.31130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ro3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 16221 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 291 | 0.2M NaCl, 0.1M Bis-Tris, 25% Polyethylene Glycol 3350, pH 5.5, EVAPORATION, temperature 291K |
