4J95
Crystal Structure of FGF Receptor 2 (FGFR2) Kinase Domain Harboring the Pathogenic K659N Mutation Responsible for an Unclassified Craniosynostosis Syndrome in Space Group C2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-16 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 278.634, 78.122, 72.791 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 37.550 - 2.377 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pvy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.376 | 2.376 |
| Number of reflections | 60839 | |
| <I/σ(I)> | 50.8 | 7.8 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES pH7.5, 25% PEG 4000, 200mM (NH4)2SO4, 3% v/v CH3CH2OH, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
