4J7B
Crystal structure of polo-like kinase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.681, 57.453, 125.736 |
| Unit cell angles | 89.15, 89.84, 72.38 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.2077 |
| Rwork | 0.205 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d5w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.606 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.210 | 2.300 |
| Number of reflections | 56677 | ||
| Completeness [%] | 94.0 | 98.3 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 50mM Bis-Tris, 100mM MOPS, 100mM NaCl, 12.5% PEG3350, pH 7.0, VAPOR DIFFUSION, temperature 293K |
