4J6G
CRYSTAL STRUCTURE OF LIGHT AND DcR3 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 149.058, 149.058, 149.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.400 |
| R-factor | 0.19591 |
| Rwork | 0.194 |
| R-free | 0.23227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4en0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.102 | 0.810 |
| Number of reflections | 43288 | |
| <I/σ(I)> | 27.2 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 0.2M SODIUM CHLORIDE, 0.1M SODIUM CITRATE:CITRIC ACID BUFFER 1.0M AMMONIUM PHOSPHATE DIBASIC, PH5.5,, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
