4J4Q
Crystal structure of active conformation of GPCR opsin stabilized by octylglucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 241.265, 241.265, 109.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.460 - 2.650 |
| R-factor | 0.21794 |
| Rwork | 0.217 |
| R-free | 0.23032 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dqb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.460 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.055 | 0.421 |
| Number of reflections | 35401 | |
| <I/σ(I)> | 17.3 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | AMMONIUM SULFATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
