4J4Q
Crystal structure of active conformation of GPCR opsin stabilized by octylglucoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 2 |
Unit cell lengths | 241.265, 241.265, 109.561 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.460 - 2.650 |
R-factor | 0.21794 |
Rwork | 0.217 |
R-free | 0.23032 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dqb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.278 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.460 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.055 | 0.421 |
Number of reflections | 35401 | |
<I/σ(I)> | 17.3 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | AMMONIUM SULFATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |