4J0E
Crystal structure of 3-hydroxyacyl-CoA dehydrogenase from Caenorhadbitis elegans in P1 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.709, 55.215, 56.066 |
| Unit cell angles | 80.45, 75.58, 72.42 |
Refinement procedure
| Resolution | 54.040 - 1.600 |
| R-factor | 0.22568 |
| Rwork | 0.223 |
| R-free | 0.27043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3had |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.224 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.040 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.367 |
| Number of reflections | 77082 | |
| <I/σ(I)> | 25.4 | 3.31 |
| Completeness [%] | 95.6 | 92.8 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 21% PEG 3350, 0.2M sodium chloride, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
