4J0E

Crystal structure of 3-hydroxyacyl-CoA dehydrogenase from Caenorhadbitis elegans in P1 space group

Summary for 4J0E

• Entry DOI: 10.2210/pdb4j0e/pdb
• Related: 4J0F
• Descriptor: Probable 3-hydroxyacyl-CoA dehydrogenase F54C8.1 (2 entities in total)
• Functional Keywords: rossmann fold, dehydrogenase, fatty acid beta-oxidation, nadh binding, mitochondrial matrix, oxidoreductase
• Biological source: Caenorhabditis elegans (nematode)
• Cellular location: Mitochondrion matrix : P34439
• Total number of polymer chains: 2
• Total formula weight: 69419.45

Authors

Xu, Y.,Sun, F.,Zhai, Y. (deposition date: 2013-01-30, release date: 2014-03-19, Last modification date: 2023-11-08)

Primary citation

Xu, Y.,Sun, F.
Purification, crystallization and preliminary crystallographic analysis of 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans.
Acta Crystallogr.,Sect.F, 69:515-519, 2013

PubMed Abstract: 3-Hydroxyacyl-CoA dehydrogenase (HAD; EC 1.1.1.35) is the enzyme that catalyzes the third step in fatty-acid β-oxidation, oxidizing the hydroxyl group of 3-hydroxyacyl-CoA to a keto group. The 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans (cHAD) was cloned, overexpressed in Escherichia coli and purified to homogeneity for crystallography. Initial crystals were obtained by the hanging-drop vapour-diffusion method. Optimization of the precipitant concentration and the pH yielded two types of well diffracting crystals with parallelepiped and cuboid shapes, respectively. Complete diffraction data sets were collected and processed from both crystal types. Preliminary crystallographic analysis indicated that the parallelepiped-shaped crystal belonged to space group P1, while the cuboid-shaped crystal belonged to space group P212121. Analyses of computed Matthews coefficient and self-rotation functions suggested that there are two cHAD molecules in one asymmetric unit in both crystals, forming identical dimers but packing in distinct manners.

PubMed: 23695566
DOI: 10.1107/S1744309113007045

Experimental method

X-RAY DIFFRACTION (1.6 Å)