4IPF
The 1.7A crystal structure of humanized Xenopus MDM2 with RO5045337
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.083, 67.493, 66.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.750 - 1.700 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.400 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.300 | 36.310 | 1.610 |
High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
Rmerge | 0.037 | 0.022 | 0.169 |
Number of reflections | 11957 | ||
<I/σ(I)> | 20.3 | 48.5 | 3.3 |
Completeness [%] | 82.6 | 99.2 | 20.5 |
Redundancy | 3.58 | 3.73 | 1.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 50% SATURATED AMMONIUM SULFATE, 100 mM BIS-TRIS, pH 6.5, 5% (V/V) PEG200, 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K |