4IPF
The 1.7A crystal structure of humanized Xenopus MDM2 with RO5045337
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.083, 67.493, 66.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.750 - 1.700 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.300 | 36.310 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.037 | 0.022 | 0.169 |
| Number of reflections | 11957 | ||
| <I/σ(I)> | 20.3 | 48.5 | 3.3 |
| Completeness [%] | 82.6 | 99.2 | 20.5 |
| Redundancy | 3.58 | 3.73 | 1.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 50% SATURATED AMMONIUM SULFATE, 100 mM BIS-TRIS, pH 6.5, 5% (V/V) PEG200, 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K |
