4IMY

The AFF4 scaffold binds human P-TEFb adjacent to HIV Tat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-09
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	100.691, 126.298, 195.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.940
R-factor	0.209
Rwork	0.207
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mi9
RMSD bond length	0.004
RMSD bond angle	0.666
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.990
High resolution limit [Å]	2.940	2.940
Rmerge		0.000
Number of reflections	54164
<I/σ(I)>	23.2	1.3
Completeness [%]	100.0	100
Redundancy	8.1	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	291	0.1 M HEPES, pH 7.0, 16% PEG 3350, 0.1% Gly-Phe, 0.1% Gly-Tyr, 0.1% Leu-Gly-Gly. Microseeding, VAPOR DIFFUSION, SITTING DROP, temperature 291K