4IJF

Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain K222A/R225A/K248A/K251A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793378
Spacegroup name	P 32 2 1
Unit cell lengths	59.704, 59.704, 67.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.104 - 2.506
R-factor	0.2026
Rwork	0.200
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fke
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.074	0.044	0.770
Number of reflections	5063
<I/σ(I)>	12.9
Completeness [%]	99.3	91.6	100
Redundancy	4	3.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298.15	0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, 30% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K