4IHF
Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 84.299, 89.441, 112.251 |
Unit cell angles | 104.06, 92.40, 118.47 |
Refinement procedure
Resolution | 43.380 - 2.100 |
R-factor | 0.168 |
Rwork | 0.168 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.050 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.103 | 0.078 | 0.402 |
Number of reflections | 154031 | ||
<I/σ(I)> | 8.2 | ||
Completeness [%] | 97.7 | 98.4 | 94.9 |
Redundancy | 3.6 | 3.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 288 | 0.1 M MES pH 6.5, 0.2 M ammonium sulfate, 20% PEG 8000, vapor diffusion, temperature 288K |