4IFD

Crystal structure of an 11-subunit eukaryotic exosome complex bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-28
Detector	PSI PILATUS 6M
Wavelength(s)	0.9199
Spacegroup name	P 1 21 1
Unit cell lengths	154.090, 107.440, 150.460
Unit cell angles	90.00, 110.63, 90.00

Refinement procedure

Resolution	49.638 - 2.805
R-factor	0.1846
Rwork	0.182
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nn6 2wp8 2ja9 2je6
RMSD bond length	0.003
RMSD bond angle	0.542
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.640	49.640	3.000
High resolution limit [Å]	2.805	20.000	2.800
Rmerge	0.176	0.069	0.548
Number of reflections	99137
<I/σ(I)>	12.25	35.76	1.89
Completeness [%]	87.4	91.2	56.5
Redundancy	12.8	15.76	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.02M Tris pH7.5, 0.05M NaCl, 0.001M MnCl2, 0.002M MgCl2, 1mM TCEP, 0.15M MES pH6.5, 0.27M NaBr, 11.4-12.2% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 292K