4IF8
Structure Of Vaspin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.550, 152.100, 61.450 |
Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
Resolution | 29.800 - 2.080 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qlp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.800 |
High resolution limit [Å] | 2.080 |
Rmerge | 0.051 |
Number of reflections | 72713 |
Completeness [%] | 99.9 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 292 | 0.1M NA CITRATE, 9% PEG4000, 0.1M Ammonia sulfate, pH 5.1, Vapor Diffusion, Hanging Drop, temperature 292K |